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Researchers uncovered a new way to clarify unsolved Mendelian diseases

A team of scientists from the National University of Singapore (NUS) led by Assistant Professor Xue Shifeng from the Department of Biological Sciences in collaboration with A*STAR, Yale-NUS, and Aix-Marseille University, and published in Nature Communications, the team discovered a new way to interpret unsolved Mendelian diseases. 

Mendelian diseases are inherited from either parent due to gene mutations in the developing egg or sperm that could result in physical defects in the newborn organism.

Mutations in the SMCHD1 gene can cause diseases such as facioscapulohumeral muscular dystrophy (FSHD), and Bosma arhinia microphthalmia syndrome (BAMS) which is a muscle degenerative disorder, and which causes abnormalities of the nose and eyes.

The discovery came about via studying the inheritance of a protein known as SMCHD1 which is coded by the SMCHD1 gene.

The researchers found that SMCHD1 from mothers controls the expression of a group of genes in offspring, known as the HOX genes, which determines the position of body parts in an embryo along the axis from its head to tail. The researchers also found that the inactivation of SMCHD1 in female zebrafish results in alterations to HOX gene expression leading to skeletal defects in their offspring.

In mammals, SMCHD1 plays a key role in X-inactivation in females, a process where one of the copies of the X chromosome is randomly selected and disabled. This makes it challenging to study the role of the SMCHD1 gene inherited from mothers because inactivating the SMCHD1 gene is lethal for female mammals.

The research team decided to use zebrafish, a vertebrate commonly used as a model organism in biomedical research, to circumvent this issue. Zebrafish lack X-inactivation, allowing the team to study the role of the SMCHD1 gene inherited from mothers. The researchers inactivated the SMCHD1 gene in zebrafish to study how it will affect gene expression and structural development in zebrafish offspring.

NUS scientists observed that SMCHD1 protein is placed into the egg by the mother. The inactivation of the SMCHD1 gene in female zebrafish caused alterations in HOX gene expression in their fertilized eggs. HOX genes play an important role in ensuring the specific patterns and identities of different body parts in the baby. The loss of the SMCHD1 gene resulted in premature activation of HOX genes resulting in skeletal patterning defects in the zebrafish offspring.

Asst Prof Xue and her team demonstrated a new concept that gene products such as proteins from the mother’s egg can control gene expression occurring in the developing embryo. Factors that control gene expression produced by the mother in the developing egg can set up the conditions for proper gene activation after the egg is fertilized with sperm. Through further lab studies, the team found that the same principle applied to mammals.

The results of the study could change the way unsolved Mendelian diseases are interpreted. Based on this study, some genetic abnormalities in parents could manifest in their children, opening possibilities of explaining birth defects seen in children by examining the genetic make-up of their parents.

Following their current study, the researchers hope to continue exploring how, at the molecular level, maternal genes control embryo gene expression after birth.

Researchers uncovered a new way to clarify unsolved Mendelian diseases

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